The second monoclonal antibodies had been ordered of Pharmingen (Hillcrest, Calif
décembre 23, 2023The second monoclonal antibodies had been ordered of Pharmingen (Hillcrest, Calif Cell staining and you can flow cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing…